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<i>Cryptococcus gattii</i> (Vanbreuseghem et Takashio) Kwon-Chung et Boekhout
<i>Cryptococcus gattii</i> (Vanbreuseghem et Takashio) Kwon-Chung et Boekhout
規(guī)格:
貨期:
編號:B233966
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Cryptococcus gattii (Vanbreuseghem et Takashio) Kwon-Chung et Boekhout
商品貨號 B233966
Deposited As Cryptococcus bacillisporus
Strain Designations A1M R265 [CBS 10514]
Alternate State Filobasidiella bacillispora Kwon-Chung, teleomorph
Application
Multigene phylogeny and phenotypic characterization.
Biomedical Research and Development Material
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Antigenic Properties Serotype B
Genotype VGIIa, AFLP6A
Preceptrol&reg; no
Genome Sequenced Strain

Yes

Mating Type alpha
Comments
Genome sequencing strain (Broad Institute, USA)
Cause cryptococcosis
Represents one of the two genotypes causing an outbreak in Vancouver Island
Multigene phylogeny and phenotypic characterization
Designated as type strain of Cryptococcus deuterogattii Hagen & Boekhout by Hagen et al. (2015)
Medium ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATACTGGACTTCGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCTTATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCAGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAATAA


D1D2 region of the 26S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGAGGAATGTGGCACTCTTCGGGGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC


26S ribosomal RNA gene and intergenic spacer (IGS), partial sequence

ATGGGAACGGGGTGCTGTAAGTGGTAGAGTAGCCTTGTTGCTACGATCCACTGAGGCTAAGCCCTTGTTCTATAGATTTGTCTCTAATATGTTAGGTCTCGGGGGGCTTCCTCTAGAGACTTGGATGTAAGAGGCTTTGCTTTTTTTTATGACGATGACTTGTCAAAGTGTGGATGACTCCAGGAGAGAGGTCGGGCTGGTAATTAGTAATATCAGTCAGTCATTTCAGCTGGCGCCATCGATACTTTATAAGTCAAACTTAACCATTTCACTGTGTAGTAAGTAGTAGGCTCTGAATTACTAGAGACACTTGCCAGGTTATCGCAAGTTGGCAGGCAGGCAGGACACACATACTATTGATTTCACGGTCATGGGGGACTTGGCTGCTGGTGCTTGAGTTGCATAATAGAGAGAGTATATGCATGTCGCGGGGGGGGGACTTGGCTAAGATGCGTTATGCGGCAAGCAAGGTAAGTAGTATTTGTATTAAAGTATTTAGTTACTTTTCCAGTCATGGGGGACTTTGATAGTGGTTGTGAGCCGGAATGAAAAATGGGTAAATGTGGTATGGATGGTGAGAGGGGGAGCTGCCAAGTGTATGCAGTGGGTGTGCTTATAGCATAGTCAAGAGGGGATGGGCAATTAAATTTTGGAAAGTTGTTTGACCCGACCTGACGGTGACTCTAATAGGCTGGTCAAGCAAACGTTTAAGTTGAGTCAAGCTCGGTCAAGCAAAGTCTAGAAAAGATTAATGATCGCTAAGAATTACTCCGGTCGCGGGGGGCTTGCAACTTGTCTGGCCCACTGTGGGTCCAGTTT


glycerol-3-phosphate dehydrogenase 1 (GPD1) gene, partial cds

TACTTGCATAAATACAATGGTTGTCAAGGTTGGAATCAACGGTTTCGGTACGTGATCTGTCTCTATCAAGCGTTGCTTCATATGTCACCAATGTGGTTTCCGATATTGTGCACCGTCCTTGGGTTACTGGTAGCTAATAGTTATGCGATAGGTCGTATCGGTCGAATTGTTCTCAGGTATGCTTTAAGTCAAGGGGTAAGACAGCTGCGTATCTAACCATCAAATTAGGAACGCCATCGAGCATGGAGACCTTGAGGTTGTTGCTGTCAACGAGTGAGTATTCTCAAAAGTGTTAATCTATTAAATAAATTATTTACTTTTGCAAAGCCCTTTCATTGACTTGGACTACATGGTATAATACTTTCTTCTGTACAATTGCTTCGTTTTGTGCTAACTGTAGCATGCGTAGGTTTACATGTTCAAGTACGACTCCGTAAGTCTCTGATGGCTCTAGCGCTCTATATGGCATTTTAACCTCATTCCCAACAGACACATGGTCGCTTCAAGGGTTCTGTCGAGGTTAAGGACGGTAAGCTCTATATCAACAACAAGGCCATTTCCGTTTTCGGTGAGAAGGACCCCGCCAACATCAAGTGGGGTGACGCTGGTGCCGAGTACATCGTTGAGTCTACCGGTGTCTTCACCACTACCGAAAAGGCCGGTGTCCAT
Name of Depositor L Hoang
Special Collection NSF - Mycology
Chain of Custody
ATCC <-- L. Hoang
Isolation
Bronchial alveolar lavage, Duncan, Vancouver Island, B.C. Canada.
Cross References

Nucleotide (GenBank) : AAFP00000000 Cryptococcus gattii VGII R265, whole genome shotgun sequencing project

References

Hu G, Kronstad JW. Gene disruption in Cryptococcus neoformans and Cryptococcus gattii by in vitro transposition. Curr Genet 49: 341-350, 2006. PubMed: 16397763

Fraser James A, et al. Same-sex mating and the origin of the Vancouver Island Cryptococcus gattii outbreak. Nature 437: 1360-1364, 2005.

Kidd Sarah E, et al. Comparative gene genealogies indicate that two clonal lineages of Cryptococcus gattii in British Columbia resemble strains from other geographical areas. Eukaryot. Cell 4: 1629-1638, 2005.

Kidd Sarah E, et al. A rare genotype of Cryptococcus gattii caused the cryptococcosis outbreak on Vancouver Island (British Columbia, Canada). Proc. Natl. Acad. Sci. USA 101: 17258-17263, 2004.

Findley K, et al. Phylogeny and phenotypic characterization of pathogenic Cryptococcus species and closely related saprobic taxa in the Tremellales. Eukaryot Cell 8(3): 353-361, 2009. PubMed: 19151324

Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262

Meyer W. et al. Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Med. Mycol. 47(6):561-570, 2009. PubMed: 19462334

D'Souza CA, et al. Genome variation in Cryptococcus gattii, an emerging pathogen of immunocompetent hosts. mBio 2: e00342-10, 2011. PubMed: 21304167

Hagen F, et al. Recognition of seven species in the Cryptococcus gattii/Cryptococcus neoformans species complex. Fungal Genet. Biol. 78: 16-48, 2015. PubMed: 25721988

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