久久精品女人天堂?V麻,久久99精品久久久久久齐齐,国产精品天干天干在线观看,?级毛片久久免费观看,国产美女在线精品免费观看,日韩欧美啪啪一区二区毛片,久久精品99国产亚洲?v成人,最新熟妇精品国产偷自在线,国产老妇多毛精品一区二区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > H2.8
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
H2.8
H2.8
規(guī)格:
貨期:
編號(hào):B164528
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 H2.8
商品貨號(hào) B164528
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue spleen
Cell Type hybridoma:lymphoblast B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age adult
Strain BALB/c
Applications
ELISA detection zona pellucida glycoprotein 2 ZP2, zona pellucida protein A
Western Blot zona pellucida glycoprotein 2 ZP2, zona pellucida protein A
detection of zona pellucida glycoprotein 2 ZP2, zona pellucida protein A
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
zona pellucida glycoprotein 2
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 10 mM HEPES, 0.15 mg/ml oxaloacetate, 0.05 mg/ml pyruvate, 0.0082 mg/ml bovine insulin and 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Subculturing Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium at 2 x 105 viable cells/mL. Add medium as the cell density increases. Maintain cultures at cell concentrations between 1 x 105 and 1 x 106 viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG1 kappa
Name of Depositor J Dean
Deposited As mouse (B cell); mouse (myeloma)
References

Animal were immunized with bacterially expressed, Hi2+-agarose purified 6-histidine tagged recombinant (amino acids 39-642) human ZP2 (zona pellucida glycoprotein 2)

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
惠州市| 霍州市| 昌邑市| 佛山市| 寿阳县| 日土县| 东港市| 普宁市| 华安县| 兴国县| 仪征市| 奎屯市| 龙陵县| 双江| 东莞市| 中西区| 都兰县| 布拖县| 望谟县| 崇左市| 新野县| 无为县| 札达县| 沁阳市| 平昌县| 兴宁市| 十堰市| 禄丰县| 会同县| 娄烦县| 中方县| 英山县| 白水县| 泗阳县| 长治县| 鸡西市| 贵南县| 和静县| 城市| 齐齐哈尔市| 德昌县|