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AP.6
AP.6
規(guī)格:
貨期:
編號:B163950
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 AP.6
商品貨號 B163950
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with the alpha chain of the AP-2 protein of all mammalian species tested.
It is useful for immunofluorescence and immunoprecipitation assays, but tends to give spurious bands in Western blots.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against AP-2 adaptor protein of clathrin coated vesicles
Cellular Products
immunoglobulin; monoclonal antibody; against AP-2 adaptor protein of clathrin coated vesicles
Comments
Animals were immunized with adaptor protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the alpha chain of the AP-2 protein of all mammalian species tested.
It is useful for immunofluorescence and immunoprecipitation assays, but tends to give spurious bands in Western blots.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing Cultures can be established by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cell density between 105 and 106 viable cells/mL.
Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1
Name of Depositor FM Brodsky
Deposited As mouse (B cell); mouse (myeloma)
References

Chin DJ, et al. 100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis. Proc. Natl. Acad. Sci. USA 86: 9289-9293, 1989. PubMed: 2574457

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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