| 產(chǎn)品名稱(chēng) |
88B |
| 商品貨號(hào) |
B163840 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with determinants on both the gamma and delta subunits of the receptor. And exhibits broad species reactivity (it reacts with receptors of mouse, rat, chicken and frog), and can be used in ultrastructural and immunofluorescence localization and in immunoprecipitation and immunoblotting procedures. |
| Derivation |
Animals were immunized with purified nicotinic acetylcholine receptor of Torpedo californica. Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against nicotinic acetylcholine receptor of Torpedo californica |
| Cellular Products |
immunoglobulin; monoclonal antibody; against nicotinic acetylcholine receptor of Torpedo californica |
| Tumorigenic |
Yes |
| Effects |
Yes, in pristane primed BALB/c mice |
| Comments |
Animals were immunized with purified nicotinic acetylcholine receptor of Torpedo californica. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with determinants on both the gamma and delta subunits of the receptor. And exhibits broad species reactivity (it reacts with receptors of mouse, rat, chicken and frog), and can be used in ultrastructural and immunofluorescence localization and in immunoprecipitation and immunoblotting procedures. The domains recognized by 88B appear to be on the cytoplasmic side of the membrane. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
SC Froehner |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Froehner SC, et al. Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor. J. Biol. Chem. 258: 7112-7120, 1983. PubMed: 6189834
Green WN, Wanamaker CP. The role of the cystine loop in acetylcholine receptor assembly. J. Biol. Chem. 272: 20945-20953, 1997. PubMed: 9252423
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