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7D4
7D4
規(guī)格:
貨期:
編號:B163828
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 7D4
商品貨號 B163828
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Antibody binds to IL-2 receptors on activated murine T and B cells.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse interleukin-2 (IL-2, interleukin 2) receptor (CD25); blocks IL-2 response but does not block binding of IL-2 to the receptor
Cellular Products
immunoglobulin; monoclonal antibody; against mouse interleukin-2 (IL-2, interleukin 2) receptor (CD25); blocks IL-2 response but does not block binding of IL-2 to the receptor
Comments
Rats were immunized with the mouse cell line HT-2.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Antibody binds to IL-2 receptors on activated murine T and B cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal:  Two to three times weekly.
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgM; kappa light chain
Name of Depositor EM Shevach
Deposited As rat (B cell); mouse (myeloma)
References

Malek TR, et al. Identification and initial characterization of a rat monoclonal antibody reactive with the murine interleukin-2 receptor-ligand complex. Proc. Natl. Acad. Sci. USA 80: 5694-5698, 1983. PubMed: 6412230

Ortega G, et al. The murine IL2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells. J. Immunol. 133: 1970-1975, 1984. PubMed: 6206144

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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