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123-10
123-10
規(guī)格:
貨期:
編號(hào):B163553
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 123-10
商品貨號(hào) B163553
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the alpha subunit and recognizes a determinant present only on the denatured enzyme.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against DNA polymerase III holoenzyme from Escherichia coli (E. coli)
Cellular Products
immunoglobulin; monoclonal antibody; against DNA polymerase III holoenzyme from Escherichia coli (E. coli)
Comments
Mice were immunized with E. coli DNA polymerase III' containing the alpha, epsilon, theta and tau subunits.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody is specific for the alpha subunit and recognizes a determinant present only on the denatured enzyme.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Iscove's modified Dulbecco's medium, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgM
Name of Depositor M Franden, CS McHenry
Deposited As mouse (B cell); mouse (myeloma)
References

Wu YH, et al. Monoclonal antibodies specific for the alpha subunit of the Escherichia coli DNA polymerase III holoenzyme. J. Biol. Chem. 259: 12117-12122, 1984. PubMed: 6090455

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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